【ACE C18色譜柱】LC-MS/MS法測定綠色蔬菜中的水溶性維生素

Water Soluble Vitamins In Green Vegetables By LC-MS/MS

ACE C18, 3μm, 100 x 2.1mm
Gradient analysis
A: 10mM ammonium acetate (aq), pH 4.5
B: 0.1% acetic acid in methanol
C: 0.3% acetic acid in methanol
Time (mins) %A %B %C
0 90 10 0
3 90 10 0
4 50 0 50
7 50 0 50
10 0 100 0
Flow rate: 0.2ml/min
Injection volume: 10μl
Column temperature: 20°C
TSQ triple quad MS; SRM mode
Detection: -ESI for vitamin C +ESI for B vitamins