【ACE C18色譜柱】普伐他汀及其異構體的LC-MS/MS分析

Pravastatin and Isomers by LC-MS/MS

All 3 compounds have MW 424
MS/MS conditions alone insufficient for selective quantitation
Baseline separation important
ACE C18 3 μm, 50 x 3.0 mm
Isocratic analysis
Acetonitrile-Methanol-THF-Water-Acetic acid (15:20:5:60:0.1)
Flow rate: 0.6 ml/min
Column temperature: Ambient
Injection volume: 2 μl
Sample: 1 μg/ml each isomer
API 3000 triple quad MS
TurboIonSpray –negative mode
Extracted ion chromatogram of MRM m/z 423.3 → 321.1