【ACE C18-AR色譜柱】啤酒花植物雌激素的LC-MS/MS分析

LC-MS/MS of phytoestrogens from hop extracts

Hop extracts obtained by pressurised liquid extraction using ASE 200 sys

ACE C18-AR, 3μm, 150 x 4.6mm
Gradient analysis
A: 1% formic acid in acetonitrile
B: 1% formic acid in methanol
C: 1% formic acid in water
D: Methanol
Time (mins) %A %B %C %D
0 56 0 44 0
8 51 5 44 0
10 51 5 44 0
17 95 5 0 0
22 95 0 0 5
Flow rate: 0.6ml/min
TSQ-Quantum triple quad ESI
Spray voltage: -4500V
Precursor ion: 355.4 [M+H]+
MRM transition ions: 179 and 299
Collision energy: 28 and 16
LOQ isoxanthohumol: 0.07μg/ml
LOQ: xanthohumol: 0.01μg/ml