【ACE C18色譜柱】離子對LC-MS/MS法測定阿糖胞苷類似物分析

Cytarabine Analogues by Ion-Pairing LC-MS/MS

ACE 3 C18 3μm, 50 x 2.1 mm
Gradient analysis
A = 0.1% perfluoropentanoicacid + 0.1% formic acid in water
B = 0.1% perfluoropentanoicacid + 0.1% formic acid in acetonitrile
T (mins) %B
0 0
0.5 0
3.0 13
4.0 90
5.0 0
Flow rate: 0.7 ml/min
API 4000 MS
TurboIonSpray, positive mode
Source temperature: 550°C
Cytarabineand cytidineare isobaric.
Robust method with good separation achieved.
LLOQ = 1 ng/ml human plasma