【ACE Excel色譜柱】人血漿中15-羥基盧比前列酮分析

15-Hydroxy Lubiprostone in Human Plasma

Lubiprostone, a fatty acid derived from prostaglandin E1, is rapidly metabolised to 15-hydroxy lubiprostone.
Quantitation is based on 15-hydroxy lubiprostone, with the d4 analogue as internal standard
ACE Excel 2 C18 2μm, 50 x 3.0mm
Isocratic analysis
A = 0.1% formic acid in water
B = Acetonitrile
Flow rate: 0.65ml/min
Column temperature: 35°C
Injection volume: 15μl
MDS Sciex API 5000
TurboIonSpray negative mode
IonSpray voltage: -4500V
Source temperature: 450°C
Transitions monitored:
15-Hydroxy lubiprostone
391.2 → 373.2
I.S. (15-Hydroxy lubiprostone-d4)
395.2 → 377.2