【ACE UltraCore SuperC18色譜柱】細胞毒性藥物的UHPLC-MS/MS分析

Cytotoxic Agents by UHPLC-MS/MS

Thermo Scientific Accela UHPLC
ACE UltraCore SuperC18, 2.5mm, 100 x 2.1mm
Gradient analysis
A = 0.1% formic acid in water
B = 0.1% formic acid in acetonitrile
Time (mins) %B
0 2
1 2
3 80
5 80
5.1 2
8 2
Flow rate: 0.25ml/min
Thermo Vantage triple quadrupole MS MRM +ve ESI mode
Spray voltage: 3500V
Nitrogen sheath and auxiliary gas CID with argon at 1.5 mTorr