【ACE Excel色譜柱】飲用水中藍藻/綠藻微囊藻毒素色譜分析

Microcystins From Blue/Green Algae In Drinking Water

Bruker Advance UHPLC system
ACE Excel 2μm C18, 100 x 2.1mm
Gradient elution
A = 0.1% formic acid in water
B = Acetonitrile
T (mins) %B T (mins) %B
0 30 7.1 30
1 30 10 30
7 95
Flow rate: 0.4mL/min
Column temperature: 40°C
Injection volume: 50μL
Concentration each microcystin: 0.05ppb
Bruker EVOQ Elite triple quad MS
VIP heated-ESI temperature: 350°C
Cone gas temperature: 200°C
Spray voltage: 4500V (+)
Collision gas: argon 1.5mTorr