Each time the ACE NH2 column is taken from storage to be used for reducing sugar analysis the following steps should be performed for optimum performance prior to commencing the analysis
1.20 column volumes of 7:3 v/v MeCN/H2O
2.50 column volumes of 7:3 v/v MeCN/H2O + 0.1% v/v ammonia solution (32% approx.)
3.20 column volumes of 7:3 v/v MeCN/H2O
Figure 1 Sugars analysis with ACE 5 NH2 (50 x 2.1 mm) before and after flushing with 0.1% v/v NH3 in 75:25 v/v MeCN/H2O, 0.21 mL/min, 35°C, RID. Peak identities: 1 = Fructose, 2 = Sucrose, 3 = Lactose
Storing the ACE NH2 column
To maximise column lifetime, flush the column with 1:1 v/v MeCN:H2O for 20 column volumes followed by 100% isopropanol for 20 column volumes before fitting the end caps and replacing in the box.